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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #125686
Title: DETECTION OF YERSINIA SPECIES IN PIG TONSILS BY A 5' NUCLEASE FLUOROGENIC (TAQMAN) PCR ASSAY SPECIFIC FOR THE 16S RRNA GENE

Author
item JOURDAN, A - 3625-30-15
item MARICHAL, M - NATL ANIM DIS CENTER
item BYL, L - IOWA STATE UNIVERSITY
item Wesley, Irene

Submitted to: Food Safety Consortium Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 9/18/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We developed and optimized a fluorogenic 5' nuclease PCR assay, which is specific for the 16S rRNA present in the majority of Yersinia species. The optimized assay uses the reporter dye VIC and the quencher dye TAMRA. The assay amplified all species of Yersinia, except Y. ruckeri, which is a fish pathogen. The assay detects 5 pg of Yersinia DNA. The assay was used to screen tonsil swabs (n = 533) collected during the National Animal Health Monitoring System (NAHMS) Swine 2000 study. Tonsil swabs were received from 101 farms from 17 states and shipped to the National Animal Disease Center for processing. Of the 553 tonsil samples screened, 61.8% (342) were positive for the 16S rRNA gene of Yersinia.