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ARS Home » Southeast Area » Stuttgart, Arkansas » Harry K. Dupree Stuttgart National Aquaculture Research Cntr » Research » Publications at this Location » Publication #126091
Title: THE EFFECT OF TEMPERATURE AND SALINITY ON THE OUTER MEMBRANE PROTEIN PROFILE OF EDWARDSIELLA TARDA.

Author
item Darwish, Ahmed
item NEWTON, JOSEPH - AUBURN UNIVERSITY
item PLUMB, JOHN - AUBURN UNIVERSITY

Submitted to: World Aquaculture Society Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/2001
Publication Date: 1/20/2002
Citation: DARWISH, A.M., NEWTON, J.C., PLUMB, J.A. THE EFFECT OF TEMPERATURE AND SALINITY ON THE OUTER MEMBRANE PROTEIN PROFILE OF EDWARDSIELLA TARDA.. WORLD AQUACULTURE SOCIETY MEETING. 2002. p.162.

Interpretive Summary:

Technical Abstract: The outer membrane proteins (OMP), prepared with sodium N-lauroyl sarcocinate from 10 fish isolates of (Edwardsiella tarda) were examined using sodium dodecyl sulfate-polyacrylamide gradient gel electrophoresis. The OMP profile of (E. tarda) (ATCC 15947; the type strain of (E. tarda)) was used as a reference. The OMP profile of the type strain (E. tarda) cultured at 25 C had four major protein bands; 40, 36.5, 34, and 28.5 kDa and a large number of minor proteins ranging in size from approximately 10 to 120 kDa. A comparison between the OMP profiles of the (E. tarda) isolates revealed differences between the isolates. The 34 or 36.5 kDa outer membrane protein bands were not consistent in five (E. tarda) isolates. Moreover, two major bands of 47 and 44 kDa were present in only two (E. tarda) isolates. The incubation temperature of 25 and 35 C had no effect on the outer membrane protein profiles of 9 out of 10 of the (E. tarda) isolates. To evaluate the effect of salinity, the 10 (E. tarda) isolates were cultured in brain heart infusion broth (BHIB) containing 0.5, 1.5 and 3.0 % sodium chloride. The reactions of (E. tarda) isolates to the different salinity levels were placed into three groups; the first group expressed more or fewer protein bands at 1.5 % sodium chloride. The second group lost major bands at 3 % salinity while the third group had no change in the OMP profile with salinity. The outer membrane protein profile differences observed between the isolates and their different reactions to salinity levels suggest that (E. tarda) is not a homogenous species.