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Title: EARLY DETECTION OF BIOTIC AND ABIOTIC STRESS BY KINETIC IMAGING OF CHLOROPHYLL FLUORESCENCE

Author
item SOUKUPOVA, JULIE - PHOTOSYN. RESEARCH CTR CZ
item SMATANOVA, S - PHOTOSYN. RESEARCH CTR CZ
item JEGOROVA, A - PHOTOSYN. RESEARCH CTR CZ
item FERIMAZOVA, H - PHOTOSYN. RESEARCH CTR CZ
item KUPPER, H - PHOTOSYN. RESEARCH CTR CZ
item SETLIK, S - PHOTOSYN. RESEARCH CTR CZ
item MALDENER - PHOTOSYN. RESERACH CTR CZ
item WHITMARSH, CLIFFORD
item TRTILEK, MARTIN - PHOTOSYN. RESEARCH CTR CZ
item NEDBAL, LADISLAV - PHOTOSYN. RESEARCH CTR CZ

Submitted to: International Congress of Photosynthesis Brisbane Australia
Publication Type: Proceedings
Publication Acceptance Date: 8/25/2001
Publication Date: 12/20/2001
Citation: Soukupoval, J., SMATANOVA, S., JEGOROVA, A., FERIMAZOVA, H., KUPPER, H., SETLIK, S., WHITMARSH, C.J., TRTILEK, M., NEDBAL, L. 2001. Early detection of biotic and abiotic stress by kinetic imaging of chlorophyll fluorescence. Proceedings: 12th International Congres on Photosynthesis. CSIRO Publishing: Melbourne, Australia. 2001.

Interpretive Summary:

Technical Abstract: Fluorescence imaging technology produces a two dimensional map of fluorescence transients that reveals differences in photosynthetic activity. Chlorophyll fluorescence imaging is used to investigate variations in photosynthesis within a single leaf to identify regions of damage or between different plants or colonies to select for mutants. Studies of biotic stress using chlorophyll fluorescence imaging revealed heterogeneous photosynthetic activities leaves that had been exposed to a pathogen. Recently we introduced a rapidly modulated measuring light based kinetic imaging fluorometer. Using this instrument we show that biotic stress can be detected by fluorescence emission heterogeneity before than any symptoms can be detected visually. For example, using images of chlorophyll fluorescence we were able to distinguish between mold-infected areas that eventually spread over the surface of the fruit, and damaged areas that do not increase in size during ripening. Other applications include monitoring biotic and abiotic damage in leaves and whole plants.