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ARS Home » Research » Publications at this Location » Publication #128201
Title: A SURVEY OF SINGLE NUCLEOTIDE POLYMORPHISMS IN GLYCINE GERMPLASM

Author
item ZHU, YOULIN - CHINA
item SONG, QIJIAN - CHINA
item CREGAN, PERRY

Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: 1/16/2002
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Single Nucleotide Polymorphisms (SNPs) are the most abundant form of DNA sequence variation, and are useful for genetic mapping, genetic diversity and evolution studies in plants. In a previous study, SNP frequency (Watterson's Theta) was 0.45 and 1.57 SNPs per kbp in coding and non-coding gene sequences, respectively, based on sequence comparisons of 25 genotypes representative of N. American cultivated soybean (Glycine max). In the current study our objective was to further define the pattern of DNA sequence variation within G. max, G. soja (wild soybean), and G. tomentella (a perennial Glycine species). Within each species germplasm was selected for maximum diversity based upon geographic origin. A total of 20 fragments including both coding and non-coding DNA was sequenced in each of 52 G. max, 24 G. soja and 20 G. tomentella genotypes. Watterson's Theta was similar in the 52 G. max genotypes to that found previously in N. American soybean. Somewhat greater sequence diversity was discovered in G. soja. In contrast, in G. tomentella sequence diversity was significantly higher than in G. max or G. soja. G. tomentella genotypes fell into two dissimilar groups that were clearly defined by those G. tomentella accessions with 38 chromosomes and those with 40 chromosomes.