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Title: DISCRIMINATION OF ALFALFA POPULATIONS FOR RESISTANCE TO APHANOMYCES EUTEICHES USING REAL TIME FLUORESCENT PCR

Author
item Vandemark, George
item GRITSENKO, M - WSU-IAREC, PROSSER, WA

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2001
Publication Date: 8/1/2001
Citation: VANDEMARK, G.J., GRITSENKO, M.A. DISCRIMINATION OF ALFALFA POPULATIONS FOR RESISTANCE TO APHANOMYCES EUTEICHES USING REAL TIME FLUORESCENT PCR. PHYTOPATHOLOGY, 91:S91. 2001.

Interpretive Summary:

Technical Abstract: Real-time quantitatitve PCR was used to discriminate between check alfalfa populations for resistance to Aphanomyces euteiches. The study included isolates of both race 1 and race 2 of the pathogen. Plants of each population were bulked into groups of 15 plants and DNA was extracted from roots of these bulked samples and also from bulked samples of roots of uninoculated plants. DNA samples were amplified with two different sets of specific primers and dual-labeled probes (TaqMan). The amount of pathogen DNA present in each sample was quantified based on the real-time detection of fluorescent amplicons. In experiments with race 1 isolates, the amount of pathogen DNA present in the resistant check WAPH-1 was significantly less than that detected in the susceptible check Saranac. In experiments with a single race 2 isolate, the amount of pathogen DNA in the resistant check WAPH-5 was significantly less than in either of the susceptible checks, Saranac and WAPH-1, but there was no significant difference betwee the two susceptible checks. No amplicons were detected in healthy roots.