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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #129773
Title: THE USE OF AFLP MARKERS FOR THE IDENTIFICATION OF CARROT BREEDING LINES ANDF1 HYBRIDS

Author
item GRZEBELUS, DARIUSZ - AG UNV KRAKOW, POLAND
item SENALIK, DOUGLAS - UNIVERSITY OF WISCONSIN
item JAGOSZ, BARBARA - AG UNIV KRAKOW, POLAND
item Simon, Philipp
item MICHALIK, BARBARA - AG UNIV KRAKOW, POLAND

Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/6/2002
Publication Date: N/A
Citation: N/A

Interpretive Summary: Carrot is a major world vegetable crop, and an important dietary source of provitamin A carotenes. Production of new cultivars utilizes hybrid breeding to improve yield and nutritional quality. Commercial production depends upon the use of F1 hybrids. It is therefore essential for seed producers to evaluate seed purity for successful production of hybrids. Identification of reliable molecular markers would facilitate this process. In this study, molecular markers were used to analyze carrots hybrid production in Poland, and to find markers useful for line identification and the evaluation of hybrid seed purity. These markers worked well in accomplishing this goal. This information is useful for carrot seed producers and growers in ascertaining seed purity.

Technical Abstract: Four inbred lines of carrot (cytoplasmic male steriles and corresponding maintainers) and eight of their F1 hybrids were studied with the AFLP technique to examine their genetic relationship and produce markers useful for testing hybrid seed purity. Eighty-six polymorphic amplicons were identified using 8 primer pair combinations. Genetic distance was estimated on the basis of presence or absence of the polymorphic bands. The dendrogram plotted on the basis of the AFLP data closely represented the pedigree relationships of the lines and their hybrids. From 1 to 6 amplicons specific for a breeding line were identified. Most of them were also present in the respective F1 hybrids, thus providing a potentially useful test of hybrid seed purity. A subset of 39 markers produced using three primer combinations was used to evaluate levels of polymorphism within two parental lines and the corresponding hybrid. Multidimensional scaling plot revealed three clusters corresponding to the studied lines and their progeny.