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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #129882
Title: AN ANALYSIS OF THE HYBRID NATURE OF SOLANUM XEDINENSE BERTH, USING MOLECULAR, CYTOLOGICAL AND CROSSABILITY STUDIES

Author
item SERQUEN, FELIX - UNIVERSITY OF WISCONSIN
item Hanneman Jr, Robert

Submitted to: Potato Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/17/2002
Publication Date: N/A
Citation: N/A

Interpretive Summary: A wild species hybrid, with 60 chromosomes, grows naturally in Mexico and is a hybrid between a wild species with 72 chromosomes and a cultivated species with 48 chromosomes. The origin of this hybrid needs to be determined, as well as used to follow or determine if introgression can or has occurred between wild and cultivated species. The hybrid nature, indicating that the proposed 72 chromosome species and one or both of the cultivated 48 chromosome species were involved, was confirmed using molecular, cytological and crossability techniques. Some unique molecular markers were noted which may allow following of introgression from cultivated to wild species populations. This is a simple method to determine introgression of cultivated genes into wild populations that simply is taking advantage of what nature has been doing. It will allow us to understand the purity of the wild species populations, and may give us better means to locate genes of interest.

Technical Abstract: Solanum xedinense Berth., a pentaploid (2n=5x=60) interspecific hybrid between the cultivated potato and the wild species Solanum demissum Likdl., represents evidence of genetic exchange between wild an cultivated species. It has been found in Central mexico at altitudes between 2000 and 3500 meters, growing along the edges of cultivated fields, irrigation ditches roadside thickets and forest margins. This stu examined the hybrid nature of S. xedinense using molecular, cytological and crossability data. RAPD and AFLP markers were used to support S. xedinense as a hybrid between the cultivated potato and S. demissum. Meiotic analysis and crossability studies were used to examine the potential of S. xedinense to form offspring. Several accessions of S. tuberosum L. subsp. tuberosum and subsp. andigena Hawkes, S. demissum and an accession of S. tuberosum and S. demissum were included for comparison. Molecular analysis positioned the natural and artificial hybrids as an intermediate group between the parental abnormal in both natural and artificial hybrids, and reduced crossability was noted for S. xedinense. Although gene flow appears to happen between wild and cultivated potato, the potential for the natural hybrids to become established may depend mostly on their ability to clonally propagate themselves.