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Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Proceedings Publication Acceptance Date: 1/1/2002 Publication Date: 5/1/2002 Citation: MIKLAS, P.N. MARKER ASSISTED SELECTION FOR DISEASE RESISTANCE IN COMMON BEAN. BEAN IMPROVEMENT COOPERATIVE ANNUAL REPORT, 45:1-3. 2002. Interpretive Summary: Technical Abstract: Marker-assisted selection can provide an effective and efficient breeding tool for detecting, tracking, retaining, combining, and pyramiding disease resistance genes (for reviews see Kelly and Miklas, 1998 and1999). For common bean, PCR-based RAPD and SCAR markers linked with more than 20 disease resistance genes have been obtained to date. Specific applications of MAS for disease resistance in common bean using some of these linked markers is detailed. The utility of many of these linkages for marker- assisted selection of the resistance gene or QTL, however, has not been demonstrated outside of the original mapping population. There are various reasons why some of the linked markers may not be useful or have restricted utility, including: i) the linkage is not tight enough, or the linkage intensity may vary widely across different genetic backgrounds due to recombination suppression, and ii) the marker is difficult to assay in certain genetic backgrounds or using different PCR protocols and equipment Once the limitations of a marker for the purpose of indirect selection have been determined, and its effective use for MAS outside the original mapping population validated, it can become an invaluable tool in disease resistance breeding as highlighted above. As more resistance-linked markers are found, characterized and mapped, the power of MAS for developing more durable and multiple disease resistant cultivars will increase substantially in dry and snap bean breeding programs across the world. |
