INCUBATION OF EGG CONTENTS POOLS FOR RAPID DETECTION OF SALMONELLA ENTERITIDIS

Authors: Gast, Richard; Holt, Peter

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2002
Publication Date: 8/11/2002
Citation: Gast, R.K., Holt, P.S. 2002. Incubation of Egg Contents Pools For Rapid Detection Of Salmonella Enteritidis. Poultry Science Association Meeting Abstract.

Interpretive Summary:

Technical Abstract: The present study determined the rates at which initial inocula of approximately 10 Salmonella enteritidis (SE) cells multiplied in 10-egg pools, some of which were supplemented with concentrated broth media or with a source of iron. At 37 C, SE levels usually grew to 1000 cells/ml within 9 hours in supplemented egg pools, but 15 hours were required to reach the same levels in unsupplemented pools. Similarly, SE multiplication to levels of 100 million cells/ml was achieved in 15 hours in most supplemented pools, but usually required 24 hours in unsupplemented pools. At 25 C, the 1000 cells/ml level was attained within 18 hours in most supplemented pools, but not until 36 hours in unsupplemented pools. Likewise, most supplemented pools contained 100 million cells/ml after 36 hours of incubation, but 63 hours were necessary to support similar bacterial multiplication without supplementation. Accordingly, the length of incubation time necessary for consistent detection of small numbers of SE in egg contents pools depends on the incubation temperature used, on whether the egg pools are supplemented to increase the rate of bacterial multiplication, and on the sensitivity of subsequent tests applied to the incubated pools.