Author
![]() |
SANOGO, SOUMAILA - UNIVERSITY OF MARYLAND |
![]() |
HEBBAR, PRAKASH - M&M MARS, INC. |
![]() |
Bowers, John |
Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only Publication Acceptance Date: 7/28/2002 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Nutritional requirements for developing formulations of Trichoderma stromaticum, a biocontrol agent for witches' broom disease on cacao, were evaluated using two carbon and three nitrogen sources mixed to provide four C:N ratios of 5:1, 10:1, 15:1, and 20:1 in liquid and solid growth media. Mineral requirements of T. stromaticum were studied using media with complete nutrient status, and media deficient in magnesium, phosphorus, potassium, or sulfur. There were interactive effects of carbon source, carbon rate, and nitrogen source on mycelial growth, conidia production, and chlamydospore formation. Conidia were produced on sucrose-amended media, but few were produced on glucose-amended media. Conversely, chlamydospores were abundantly produced on glucose-amended media compared to sucrose-amended media. Few conidia were formed on media lacking phosphorus. Results from these nutritional studies should provide crucial information for efficient procedures for fermentation and formulation of T. stromaticum. |