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Title: AGE DIFFERENCE IN LYMPHOCYTE PROLIFERATION AND IL-12 AND IFN-Y PRODUCTION FOLLOWING SALMONELLA ENTERITIDIS VACCINATION

Author
item OKAMURA, MASASHI - 1265-40-00
item Lillehoj, Hyun
item RAYBOURNE, RICHARD - FDA
item HECKERT, ROBERT - VA-MD REG. COL. VET. MED.
item BABU, UMA - FDA

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2002
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The present study was conducted to investigate the effect of age on cell-mediated immune responses to different antigens from Salmonella serovar Enteritidis (SE) following vaccination with the commercially available heat-the killed bacterin. Eight-month- and 4-week-old chickens were given two subcutaneous injections with SE bacterin at 2 weeks apart. At 4, 7, 11, 14, 18, and 21 days post immunization (PI), spleen lymphocytes were stimulated with Concanavalin (Con) A, heat-killed SE (HK-SE), LPS, flagella, outer membrane protein (OMP), and porin and their proliferation measured using a non-radioactive proliferation assay (?CCK-8). Interferon ???IFN-g) and interleukin-2 (IL-2) produced by spleen cells stimulated with different SE antigens and secreted in the sera were assessed using a ELISA using antigen-specific monoclonal antibodies. Vaccinated chickens of 4-week of age (VY) showed higher proliferation response to LPS and flagella antigen, but not to the other antigens, compared to the unvaccinated young chickens, whereas 8-month-old chickens showed reduced proliferation response to SE antigens. Serum IFN-? and IL-2 levels were higher in the vaccinated birds compared to the age-matched control chickens regardless of the age group. The levels of IFN-? produced by the SE antigen-stimulated spleen cells were higher in the vaccinated young birds compared to the old chickens. IFN-? level in sera showed a significant increase after vaccination which corresponded to high IFN-? production by splenic cells stimulated with Con A and SE antigens. These results indicate that various antigenic components of SE bacteria induce IFN-? and IL-2 production, and younger chickens show better T-lymphocyte-mediated immunity following SE vaccination