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Title: PHYLOGENETIC AND SEROLOGIC ANALYSIS OF RUMINANT ATADENOVIRUSES

Author
item LEHMKUHL, HOWARD
item HOBBS, LEA

Submitted to: International Congress of Virology
Publication Type: Proceedings
Publication Acceptance Date: 5/28/2002
Publication Date: 7/20/2002
Citation: LEHMKUHL, H.D., HOBBS, L.A. PHYLOGENETIC AND SEROLOGIC ANALYSIS OF RUMINANT ATADENOVIRUSES. XIIth INTERNATIONAL CONGRESS OF VIROLOGY. 2002. Abstract p. 311, #V-925.

Interpretive Summary:

Technical Abstract: The Adenoviridae family was originally divided into two genera, Mastadenovirus and Aviadenovirus. Because some of the bovine adenoviruses (BAdV) lacked the Mastadenovirus genus specific antigen and were biologically different, the bovine adenoviruses were divided into two subgroups. Division was further supported after genomic analysis demonstrated marked differences in the two subgroups. A new genus, Atadenovirus, within the family Adenoviridae has now been created to accommodate this unique adenovirus cluster. The subgroup 2 BAdVs (BAdV-4 through -8) has been assigned to the new Atadenovirus genus. Ovine adenovirus isolate 287 (OAdV-7) has been proposed as the prototype species for the Atadenovirus genus because it was the first of these unique viruses to have the complete genome sequenced. The objectives of this study were to compare antigenic cross reactivity and hexon gene sequence of known and putative members of the Atadenovirus genus. Results of reciprocal cross-neutralization tests demonstrated antigenic relationships in either one or both directions among BAdV-6, -7, OAdV-7, goat adenovirus (GAdV-1), and a deer adenovirus OdAdV. Restriction digests of cloned 2,700 bp hexon PCR amplicons produced type specific patterns. Phylogenetic analysis of the amino acid sequences clustered members of the Atadenovirus genus on a separate branch from the Mastadenovirus and Aviadenovirus genera. Classification of BAdV-4 through -8, GAdV-1 and OdAdV-1 as members of the Atadenovirus genus was confirmed based on phylogenetic analysis of the hexon amino acid sequence.