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Title: COMPARISON OF DNA EXTRACTION METHODS FOR GENETIC ANALYSIS OF GARLIC

Author
item HARRIS, JACKIE - USDA-ARS-NCGRP
item Henk, Adam
item Volk, Gayle

Submitted to: Colorado State University Undergraduate Research: Creative Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 3/29/2002
Publication Date: 4/23/2002
Citation: Harris, J., Henk, A.D., Volk, G.M. Comparison of dna extraction methods for genetic analysis of garlic. Colorado State University Undergraduate Research: Creative Symposium, April 23, 2002, Fort Collins, Colorado. p.60.

Interpretive Summary: Garlic is an expensive crop to maintain in the National Plant Germplasm System (NPGS) since bulbs must be harvested and re-planted in the field each year. If duplicate accessions were eliminated, then the cost of maintaining this collection would be lower. Amplified Fragment Length Polymorphisms (AFLP) will be used to identify duplicate garlic accessions in the NPGS garlic collection. The purpose of this project was to determine the most desirable DNA extraction method for garlic by comparing the time input, quality and quantity of DNA, and cost efficiency of 2 Qiagen DNA prep kits. The first kit was the DNeasy Plant Mini Kit that required manual grinding for each individual sample. The second kit was the DNeasy 96 well kit that was performed in a 96 well format. We determined that the high-throughput 96-well format DNA extraction method was preferable for our AFLP analysis.

Technical Abstract: Garlic is an expensive crop to maintain in the National Plant Germplasm System (NPGS) since bulbs must be harvested and re-planted in the field each year. If duplicate accessions were eliminated, then the cost of maintaining this collection would be lower. Amplified Fragment Length Polymorphisms (AFLP) will be used to identify duplicate garlic accessions in the NPGS garlic collection. The purpose of this project was to determine the most desirable DNA extraction method for garlic by comparing the time input, quality and quantity of DNA, and cost efficiency of 2 Qiagen DNA prep kits. The first kit was the DNeasy Plant Mini Kit that required manual grinding for each individual sample. The second kit was the DNeasy 96 well kit that was performed in a 96 well format. We determined that the high-throughput 96-well format DNA extraction method was preferable for our AFLP analysis.