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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Insect Genetics and Biochemistry Research » Research » Publications at this Location » Publication #134707
Title: MOLECULAR PHYLOGENETIC RELATIONSHIPS AMONG DIABROTICA SPECIES (ACCESSION NO. AF195202)

Author
item Roehrdanz, Richard

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: 5/11/2001
Publication Date: 5/11/2001
Citation: Roehrdanz, R.L. 2001. Molecular phylogenetic relationships among Diabrotica species. GenBank. Accession no. AF195202.

Interpretive Summary:

Technical Abstract: Corn rootworms of the genus Diabrotica (Coleoptera: Chrysomelidae) are the most serious pest of corn in midwestern United States. Despite their economic importance, phylogenetic relationships within the genus remain unclear. Phylogenetic analysis of five Diabrotica was undertaken using DNA sequences of the nuclear rDNA first internal transcribed spacer region (ITS1) and a portion of the mtDNA cytochrome oxidase I and II genes (COI/COII). Parsimony and neighbor-joining cladograms indicated that southern corn rootworm is sister to banded cucumber beetle, whereas, northern corn rootworm forms a distinct clade with western and Mexican corn rootworm. ITS1 and COI/COII were found to be useful markers for determining phylogenetic relationships among diabroticites.