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Title: "CULTIVATION OF ENDOSYMBIONT BACTERIA IN A BEMISIA TABACI B BIOTYPE CELL LINE".

Author
item McKenzie, Cindy
item Hunter, Wayne
item BROWN, J. K. - UNIV. OF ARIZONA
item SINISTERRA, X. - UNIV. OF FLORIDA
item SASAKI, TETSUHIKO - UNIV. OF TOKYO
item Shatters, Robert - Bob

Submitted to: Florida Entomological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 6/6/2002
Publication Date: 7/25/2002
Citation: Mckenzie, C.L., Hunter, W.B., Brown, J., Sinisterra, X., Sasaki, T., Shatters, R.G. 2002. "cultivation of endosymbiont bacteria in a bemisia tabaci b biotype cell line".. Florida Entomological Society Annual Meeting.

Interpretive Summary:

Technical Abstract: A complement of eubacterial endosymbionts were cultivated in a whitefly cell line. Endosymbiont identification was verified using diagnostic PCR, DNA sequencing, Western Blot analysis and real time RT-PCR. This is the first report of in-vitro cultivation of at least two of these arthropod endosymbionts in an insect cell line. Molecular analysis of these endosymbionts and the associated reproductive alterations found in natural arthropod hosts should be facilitated by the development of an in vitro culture system and will aid in determining the influence of these organisms on insect biology.