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Title: RECESSIVE AND DOMINANT MUTATIONS IN THE ETHYLENE BIOSYNTHETIC GENE ACS5 OF ARABIDOPSIS CONFER CYTOKININ INSENSITIVITY AND ETHYLENE OVERPRODUCTION, RESPECTIVELY

Author
item VOGEL, J - UNIV OF ILLINOIS
item WOESTE, K - UNIV OF ILLINOIS
item Theologis, Athanasios
item KIEBER, J - UNIV OF ILLINOIS

Submitted to: Proceedings of the National Academy of Sciences (PNAS)
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/14/1998
Publication Date: N/A
Citation: Vogel, J.P., Woeste, K.E., Theologis, A., Kieber, J.J. 1998. Recessive and dominant mutations in the ethylene biosynthetic gene acs5 of arabidopsis confer cytokinin insensitivity and ethylene overproduction, respectively. Proceedings of the National Academy of Sciences, 95(8) 4766-4771.

Interpretive Summary: Recessive and dominant mutations in the ethylene biosynthetic gene ACS5 of Arabidopsis confer cytokinin insensitivity and ethylene overproduction, respectively.

Technical Abstract: We identified a set of cytokinin-insensitive mutants by using a screen based on the ethylene-mediated triple response observed after treatment with low levels of cytokinins. One group of these mutants disrupts ACS5, a member of the Arabidopsis gene family that encodes 1-aminocyclopropane-1-carboxylate synthase, the first enzyme in ethylene biosynthesis. The ACS5 isoform is mainly responsible for the sustained rise in ethylene biosynthesis observed in response to low levels of cytokinin and appears to be regulated primarily by a posttranscriptional mechanism. Furthermore, the dominant ethylene-overproducing mutant eto2 was found to be the result of an alteration of the carboxy terminus of ACS5, suggesting that this domain acts as a negative regulator of ACS5 function.