Author
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LYNN, DWIGHT |
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Submitted to: Journal of Insect Science
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 5/2/2002 Publication Date: 5/20/2002 Citation: Lynn, D.E. 2002. The methods for maintaining insect cell cultures. Journal of Insect Science. Interpretive Summary: Insect cell lines are commonly used in research and product development by hundreds of facilities (government, university and industry) around the world. Because of their wide and expanding use, a uniform method for maintaining continuous cultures is needed. The three parts of this paper describe methods that can be used for different cell types in culture; loosely attached, attached, and firmly attached. The methods are based on twenty years of experience by the author and will be freely available on a public web site for use by all researchers. Technical Abstract: Insect cell cultures are now commonly used in insect physiology, developmental biology, pathology, and molecular biology. As the field has advanced from a methods development to a standard procedure, so has the diversity of scientists using the technique. This paper describes techniques that are effective for maintaining various insect cell lines. The procedures are differentiated between loosely or non-attached cell strains, attached cell strains, and strongly adherent cell strains. |
