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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #136510
Title: A BARLEY CONSENSUS MAP INTEGRATING SSR, RFLP, AND AFLP MARKERS

Author
item KARAKOUSIS, A - UNIV OF ADELAIDE
item Gustafson, J
item CHALMERS, K - UNIV OF ADELAIDE
item BARR, A - UNIV OF ADELAIDE
item LANGRIDGE, P - UNIV OF ADELAIDE

Submitted to: Australian Journal of Agricultural Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/1/2003
Publication Date: 12/15/2003
Citation: Karakousis, A., Gustafson, J.P., Chalmers, K.J., Barr, A.R., Langridge, P. 2003. A barley consensus map integrating SSR, RFLP, and AFLP markers. Australian Journal of Agricultural Research. 54(12):1173-1185.

Interpretive Summary: In the past, it has been difficult to accurately determine the location of many types of barley molecular markers due to the lack of commonality between international barley linkage maps. In this study, a consensus map was constructed to provide a quick method of aligning and comparing barley linkage maps. Unlike previous consensus maps, the purpose of this map is to provide a tool for scientists to accurately locate molecular markers to chromosome regions of interest. It is estimated that markers placed on the consensus map are located very close to their true positions as determined by the cross-examination of five Australian linkage maps. The consensus map is currently being used to identify markers closely linked to barley agronomic traits. It is envisaged that the consensus map will benefit small-grain researchers by providing an efficient means of choosing markers of interest for future genetic or plant breeding studies on a worldwide basis.

Technical Abstract: A consensus map of barley combining SSR, RFLP, and AFLP markers has been developed by combining five Australian barley linkage maps, `Galleon x Harruna Nijo', `Chebec x Harrington', `Clipper x Sahara', `Alexis x Sloop', and `Amaji Nijo x WI2585' using the software package JOINMAP 2.0. The new consensus map consists of 700 markers, with 138 being SSRs, and has a total genetic distance of 933 cM. The consensus map order appears to be in good agreement with the Australian barley linkage maps with the exception of a small inversion located close to the centromere of chromosome 5H. Similarly, the SSR map orders are in good agreement with SSR markers integrated into the doubled-haploid linkage map in `Lina' x `Hordeum spontaneum' developed by Ramsay et al. 2000. The new consensus map provides a framework to cross examine and align partial and complete barley linkage maps using markers common to many barley maps. This map will allow researchers to rapidly and accurately select SSR markers in chromosome regions of interest for barley genetic and plant breeding studies.