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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #137090
Title: ASSSOCIATION BETWEEN LOCI WITH DELETERIOUS ALLELES AND DISTORTED SEX RATIOS IN AN INBRED LINE OF TILAPIA (OREOCHOMIS AUREUS)

Author
item AVTALION, R - BAR LLAN UNIVER. ISRAEL
item HULATA, G - ARS, ISRAEL
item CNAANI, KOROL - BAR LLAN UNIVER. ISRAEL
item Palti, Yniv
item SHIRAK, A - BAR LLAN UNVER. ISRAEL

Submitted to: Journal of Heredity
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/1/2002
Publication Date: 8/1/2002
Citation: Avtalion, R.R., Hulata, G., Cnaani, K.A., Palti, Y., Shirak, A. 2002. Asssociation between loci with deleterious alleles and distorted sex ratios in an inbred line of tilapia (oreochomis aureus). Journal of Heredity. 93(4):270-276.

Interpretive Summary: Microsatellite DNA markers have recently become available for fish genetics research. In this study their utility for a whole genome scan in tilapia is demonstrated. A highly inbred line is used to demonstrate the efficiency of microsatellite for screening the genome for regions that contain genes with deleterious alleles. A detailed a statistical analysis reveals that the deleterious effects are sex specific and are leading to sex ratio distortions in this tilapia population.

Technical Abstract: Three microsatellite markers (UNH159, UNH231, and UNH216) were examined for association with both deleterious genes and sex ratio distortions in a full-sib family of 222 progeny from the fourth generation of a meiogynogenetic tilapia line (Oreochromis aureus). The three markers previously were mapped to different linkage groups and shown to be associated with genes with deleterious alleles in this line. A restricted maximum likelihood model was used for analysis of major effects and their interactions on sex ratio and viability. This model was based on selective mortality of genders ignoring effects of possible sex determining genes. The results showed that deleterious genes linked to UNH216 and UNH231 exert higher lethality in females than in males (p<0.0005 and p<0.05, respectively). UNH159 was not associated directly with sex ratio distortion, but acts strongly as a modifier of sex ratio in combination with UNH216 and UNH231. Each of the three loci was found to have a significant effect on viability (p<0.05) in the maximum likelihood analysis. The deleterious single-locus effects act strongly against females, while most of the epistatic interactions exert higher lethality in males. This contradiction results in a close to 1:1 sex ratio at maturity. The genetic mechanism and significance of such balance between genders are still unknown. A detailed analysis of sex-specific lethality may be applied by screening in appropriate series of matings and fine mapping with additional markers. Our data suggest that UNH216 and UNH231 are linked to sex ratio distortion genes and that UNH159 may be linked to a modifier of these genes.