Skip to main content
ARS Home » Research » Publications at this Location » Publication #137779
Title: FLOW CYTOMETRIC EVALUATION OF THE EFFECTS OF SODIUM BUTYRATE ON APOPTOSIS OF BOVINE NEUTROPHILS

Author
item WORKU, M - NC AG TECH U. NC
item CAMPBELL, K - NC AG TECH U. NC
item Paape, Max

Submitted to: American Dairy Science Association Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/15/2002
Publication Date: 9/12/2002
Citation: Worku, M., Campbell, K., Paape, M.J. 2002. Flow cytometric evaluation of the effects of sodium butyrate on apoptosis of bovine neutrophils [abstract). American Diayr Science Association Abstracts.

Interpretive Summary:

Technical Abstract: Bovine blood neutrophils(PMN) from four clinically healthy cows were suspended in Dulbecco's minimal essential medium and incubated at 37C for 0, 2 or 6 hours in medium alone, with 100ng/ml bacterial endotoxin, 160nM sodium butyrate, 16uM actinomycin D, mixture of sodium butyrate and bacterial endotoxin or a mixture of sodium butyrate and actinomycin D. To label necrotic and apoptotic cells, PMN were resuspended in incubation buffer containing equal proportions of propidium iodide and annexin-V-FITC and incubated in the dark at 4C for 10 minutes. Samples were then analyzed by flow cytometry. The percentage of electronically gated PMN with associated green fluorescence was used as an indicator of apoptosis. The results were analyzed using SAS statistical analysis software. Compared to PMN maintained in medium alone (11 percent), both sodium butyrate and actinomycin D slightly increased the proportion of apoptotic PMN (15 percent). Bacterial endotoxin significantly increased apoptosis of bovine PMN (27 percent). This increase was inhibited in the presence of sodium butyrate (18 percent). Sodium butyrate did not significantly affect the response to actinomycin D (15 percent). Apoptosis of bovine PMN in response to bacterial endotoxin can be inhibited by the action of sodium butyrate.