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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #138065
Title: IN PLANTA AGRIONFECTION BY CANADIAN AND GERMAN POTATO LEAFROLL VIRUS FULL-LENGTH CDNAS

Author
item KAWCHUK, L - AGRI-FOOD CANADA
item JAAG, H - MAX PLANK INSTITUT
item TOOHEY, K - AGRI-FOOD CANADA
item Martin, Robert
item RHODE, W - MAX PLANK INSTITUT
item PRUFER, D - FRAUNHOFER INSTITUT

Submitted to: Canadian Journal of Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/25/2002
Publication Date: 7/1/2002
Citation: N/A

Interpretive Summary: The production of full-length clones of potato leafroll virus strains that produce symptoms of different severity allows for the identification of genes and parts of genes that are involved in symptom expression in plants. The Canadian isolate reached lower concentration in plants and produced milder symptoms that the German isolate. Full-length clones of both isolates cause a potato leafroll disease that is indistinquishable from the disease caused by the original virus. Agroinfection with these clones led to a typical systemic infection and virus particles appeared normal when examined with an electron microscope.

Technical Abstract: Full-length infectious cDNA clones of Canadain (PLRV-flc)and German (PLRV-flg) Potato leafroll virus isolates were constructed and their biological activities examined in planta. For both constructs, agroinfection by PLRV cDNA resulted in virus mulitplication in the primary leaf followed by systemic movement and the production of icosohedral PLRV particlees. Differences between the two constructs occurred with respect to infection, movement, and titres of infectious virus produced from the two full-length cDNAs. This study reports the first PLRV agroinfection and comparison of biological characteristics between two independent infectious Luteoviridae cDNA clones.