Author
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ROBERTSON, NANCY |
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Submitted to: Phytopathology
Publication Type: Abstract Only Publication Acceptance Date: 3/2/2002 Publication Date: 7/27/2002 Citation: Robertson, N.L. 2002. Plant viruses detected in Alaskan Streptopus amplexifolius. Phytopathology.92:S69. Publication no. P-2002-0504-AMA. Interpretive Summary: Technical Abstract: Streptopus amplexifolius is a member of the Liliaceae plant family that is indigenous to south central Alaska, growing in woods and meadows. In late June 2001, plants that bordered a trail in the Denali State Park, had leaves with uncharacteristic yellow-green dashes on their leaves. A month later, similar appearing plants were found about 50 miles away near Skwentna. Leaf samples were collected from several plants from each site and processed for virus isolation. Protein extracts from the preparations of the Denali and Skwentna plants consisted of unique proteins about 33kDa and 29kDa, respectively. Long flexuous rods were detected from leaf dips with the electron microscope. Leaf sap and purified preparations were tested against universal potyvirus antiserum (Agdia) resulting in strong reactions for the Denali plants and none from the Skwentna plants. A RT- PCR detection assay using universal primers for polyvirus detection gave the predicted product for only the Denali plants. It is concluded that plants from both sites contained plant viruses that were different from each other, and that the Denali virus is a member of the family Potyviridae. This is the first report of plant viruses found in S. amplexifoilus. |
