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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #142192
Title: COMPARATIVE DNA SEQUENCE ANALYSIS OF WHEAT AND RICE GENOMES

Author
item SORRELLS, M - CORNELL UNIVERSITY
item LA ROTA, C - CORNELL UNIVERSITY
item BERMUDEZ, C - CORNELL UNIVERSITY
item GREENE, R - CORNELL UNIVERSITY
item KANTETY, R - CORNELL UNIVERSITY
item MIFTAHUDIN - UNIV OF MISSOURI
item MAHMOUD, A - UNIV OF MISSOURI
item GUSTAFSON, J
item QI, L - KANSAS STATE UNIV
item ECHALIER, B - KANSAS STATE UNIV

Submitted to: Genome Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/12/2003
Publication Date: 8/1/2003
Citation: Sorrells, M.E., La Rota, C.M., Bermudez, C.E., Greene, R.A., Kantety, R., Miftahudin, Mahmoud, A., Gustafson, J.P., Qi, L.L., Echalier, B. 2003. Comparative DNA sequence analysis of wheat and rice genomes. Genome Research. 13(8):1818-1827.

Interpretive Summary: A comparison of the evolutionary changes that have taken place between wheat and rice has not been attempted. The present study was designed to compare the DNA differences between 2835 wheat-expressed sequences and the public rice database. An understanding of how wheat and rice genes have evolved and changed over time will be of considerable value in research and breeding programs designed to utilize wheat and rice genes for both wheat and rice improvement. We analyzed the wheat genomes based on the gene order and location in the rice genome. The results revealed several spectacular evolutionary rearrangements between the gene order and location within the wheat and rice genomes. This information will be of value to cereal evolutionists, and to those scientists that are using rice sequences to locate and tag sequences in wheat for improved production.

Technical Abstract: The use of DNA sequence-based comparative genomics for evolutionary studies and for transferring information from model species to crop species has revolutionized molecular genetics and crop improvement strategies. This study compares 2835 ESTs that were physically mapped in wheat chromosome bins to the public rice genome sequence data from 2251 ordered BAC/PAC clones using BLAST. A rice genome view of homologous wheat genome locations based on comparative sequence analysis revealed numerous chromosomal rearrangements that will significantly complicate the use of rice as a model for cross-species transfer of information in non-conserved regions.