ANALYSIS OF SCLEROTIUM CEPIVORUM BY RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ASSAY

Authors: PEREZ, L - CINVESTAV DEL IPN, MEXICO; OLALDE, V - CINVESTAV DEL IPN, MEXICO; Vandemark, George; MARTINEZ, O - CINVESTAV DEL IPN, MEXICO; MARTINEZ, J - CINVESTAV DEL IPN, MEXICO; ENTWISTLE, A - CINVESTAV DEL IPN, MEXICO; LARA, J - CINVESTAV DEL IPN, MEXICO; VASQUEZ, G - CINVESTAV DEL IPN, MEXICO

Submitted to: Sixth International Workshop on Allium White Rot
Publication Type: Proceedings
Publication Acceptance Date: 3/1/1998
Publication Date: N/A
Citation: PEREZ, L., OLALDE, V., VANDEMARK, G.J., MARTINEZ, O., MARTINEZ, J., ENTWISTLE, A., LARA, J., VASQUEZ, G. ANALYSIS OF SCLEROTIUM CEPIVORUM BY RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ASSAY. PROCEEDINGS OF THE SIXTH INTERNATIONAL WORKSHOP ON ALLIUM WHITE ROT, P. 3-13. 1998.

Interpretive Summary:

Technical Abstract: Sclerotium cepivorum Berk. is the causal agent of `white rot¿ diseases of several Allium species, including onions and leeks. Genetic relationships among isolates of S. cepivorum from different regions of Mexico and also from Spain and Venezuela were assessed based on analysis of randomly amplified polymorphic DNA (RAPDs). Five different oligonucleotide primers were used to detect genetic polymorphisms among 31 isolates from the three countries. A total of 57 marker loci, all of which were polymorphic, were amplified among the isolates. A dendrogram based on pair-wise dissimilarities indicated that the Mexican isolates formed two distinct groups. One group consisted of the majority of the isolates collected from a single field. The other group included isolates collected from different regions of Mexico and the isolate from Venezuela. The isolate from Spain occupied a unique terminal branch on the dendrogram, suggesting it was the most divergent of the isolates studied. These results indicate that considerable genetic diversity is present among different isolates of S. cepivorum in Mexico.