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ARS Home » Plains Area » Houston, Texas » Children's Nutrition Research Center » Research » Publications at this Location » Publication #143855
Title: {U-13C]GLUCOSE MIDA PROVIDES ACCURATE MEASURES OF GLUCONEOGENESIS, IS EASY TO PERFORM AND REQUIRES ONLY SMALL BLOOD SAMPLE VOLUMES

Author
item Sunehag, Agneta
item CLARKE, LUCINDA - BAYLOR COLLEGE OF MEDICIN
item Bier, Dennis
item Haymond, Morey

Submitted to: Diabetes
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2001
Publication Date: 6/1/2001
Citation: SUNEHAG, A., CLARKE, L., BIER, D.M., HAYMOND, M. {U-13C]GLUCOSE MIDA PROVIDES ACCURATE MEASURES OF GLUCONEOGENESIS, IS EASY TO PERFORM AND REQUIRES ONLY SMALL BLOOD SAMPLE VOLUMES. DIABETES. 2001.

Interpretive Summary: Not required.

Technical Abstract: Background: The validity of recent in vivo methods to measure gluconeogenesis has been debated. The deuteriumoxide method with measurements of deuterium enrichment at glucose C5 is a straightforward approach to measure gluconeogenesis but is very time consuming and requires blood sample volumes which preclude its use in infants and children. The present study was undertaken to compare [U-13C]glucose MIDA, which is easy to perform and requires very small sample volumes, with that of the deuterium oxide glucose C5 method. Methods: 5 healthy male adults (age 28±3 y, weight 71±4 kg, BMI 23±1 kg/m2) (mean ± SE) were studied on two occasions. On each occasion, 8 h tracer infusions were begun 58 h into the fast. On one occasion, gluconeogenesis and glucose production were measured by [U-13C]glucose (study I), and on the other gluconeogenesis was estimated by the deuterium oxide C5 method and glucose production by [6,6-2H2]glucose (study II). Deuterium oxide (a total of 3 g/kg) was administered as 4 oral doses given at 2 h intervals beginning 48 h into the fast. Results: All data represent mean±SE of 5 measurements obtained between study h 7 and 8 (fasting h 64-65). No differences were observed between the two study occasions in plasma glucose, 4.0±0.2 (study I) vs. 4.0± 0.2 mM(study II) (ns), plasma glucose appearance rates, 6.8± 0.3(study I) and 6.9±0.2 micromol/kg min (study II) (ns) or glucose production rates, 5.8±0.3 (study I) and 6.0±0.3 micromol/kg min (study II) (ns). The estimates of gluconeogenesis provided by the two methods did also not differ significantly. Thus, the gluconeogenic rate measures by {U-13C]glucose MIDA was 5.1±0.2 and by deuteriumoxide glucose C5, 5.7±0.3 micromol/kg min (ns) i.e. gluconeogenesis accounted for 87±2 and 96±5% of GPR, respectively (ns). Conclusion: The [U-13C]glucose MIDA and the deuteriumoxide glucose C5 method provided similar estimates of gluconeogenesis. Additionally, the [U-13C]glucose measurements are highly reproducible. Thus, [U-13C]glucose MIDA provides a robust approach to measure gluconeogenesis, which can be easily and safely applied to all groups of subjects including very premature infants.