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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #146485
Title: A GENETIC MAP OF MOLECULAR MARKERS IN TRITICOSECALE WITTMACK

Author
item GONZALEZ, JUAN - UNIV. OF MADRID
item GUSTAFSON, J
item JOUVE, N - UNIV. OF MADRID
item MUNIZ, L - UNIV. OF MADRID

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2002
Publication Date: 6/30/2002
Citation: Gonzalez, J.M., Gustafson, J.P., Jouve, N., Muniz, L.M. 2002. A genetic map of molecular markers in triticosecale wittmack [abstract]. International Triticale Symposium. p. 85-93.

Interpretive Summary:

Technical Abstract: A genetic map of triticale produced with polymorphic markers based on PCR technology (RAPD, RAMP, AFLP and SSR) is reported. These revealed levels of polymorphism of 7.6%, 6.2%, 7.9% and 28.4%, respectively. The plant materials employed were two hexaploid triticale cultivars, 'Torote' and 'Presto', and 73 double-haploid lines (dh) obtained by in vitro culture of anthers (androgenesis) from F1 hybrids between the two varieties. Of the 436 molecular markers analyzed, 385 (88.3%) were located in the map. A series of markers showed distorted segregation in the androgenetic progenies (29%). The major proportion of these belonged to 'Presto' (67%). Thirty-six linkage groups were constructed. The distribution of the markers was not homogeneous among the three genomes, with 47.1% detected in the R genome. The least covered genome, A, had a smaller map with only 59 markers and 474.1 cM. Genome B included 79 markers covering a total length of 786.2 cM. The complete map reached a total length of 2647.3 cM. The mean distance between the markers was 6.9 cM.