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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #147256
Title: IDENTIFICATION OF QTLS ASSOCIATED WITH FUSARIUM HEAD BLIGHT RESISTANCE IN ZHEDAR 2 BARLEY

Author
item Dahleen, Lynn
item AGRAMA, H - NDSU
item HORSLEY, R - NDSU
item STEFFENSON, B - UNIV OF MINNESOTA
item SCHWARZ, P - NDSU
item MESFIN, A - UNIV OF MINNESOTA
item FRANCKOWIAK, J - NDSU

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/2/2003
Publication Date: 11/20/2003
Citation: DAHLEEN, L.S., AGRAMA, H.A., HORSLEY, R.D., STEFFENSON, B.J., SCHWARZ, P.B., MESFIN, A., FRANCKOWIAK, J.D. IDENTIFICATION OF QTLS ASSOCIATED WITH FUSARIUM HEAD BLIGHT RESISTANCE IN ZHEDAR 2 BARLEY. THEORETICAL AND APPLIED GENETICS. 2003. VOL. 108:95-104.

Interpretive Summary: Fusarium head blight (FHB) in barley and wheat, caused by Fusarium graminearum, is a continual problem worldwide, reducing yield and quality and producing the toxin deoxynivalenol (DON), which can affect food safety. The objective of this research was to locate barley chromosome regions that are associated with reduced FHB and DON concentrations from the Chinese cultivar Zhedar 2. DNA markers for the seven barley were developed into a linkage map, and a population of 75 segregating lines developed from crosses with Zhedar 2 were used to locate important regions. We identified nine regions associated with lower FHB severity and five for low DON, many of which were located in regions identified in previous studies. Regions that appear to be promising candidates for use in breeding and further genetic analysis include two FHB regions on chromosome 2H and one on 6H, which were also associated with low DON and later heading date in multiple environments. This study provides a starting point for manipulating Zhedar 2-derived resistance in barley to develop cultivars that will show effective resistance under disease pressure.

Technical Abstract: Fusarium head blight (FHB) in barley and wheat, caused by Fusarium graminearum, is a continual problem worldwide, reducing yield and quality and producing the toxin deoxynivalenol (DON), which can affect food safety. The objective of this research was to identify barley quantitative trait loci (QTLs) for FHB severity, DON level and related traits heading date (HD) and plant height (HT) in a segregating population of seventy-five double haploid lines, developed from the three-way cross Zhedar 2/ND9712//Foster. A linkage map of 214 RFLP, SSR and AFLP markers was constructed. Phenotypic data were collected in replicated field trials from five environments. Because of the presence of QTL x environment interactions, QTLs were identified in single environments. We identified nine QTLs for FHB severity and five for low DON, many of which were coincident with FHB QTLs identified in previous studies. Only two of the QTLs identified in this study were consistent across all five environments, and both were Zhedar 2 specific. Five of the FHB QTLs were associated with HD, and two were associated with HT. Regions that appear to be promising candidates for MAS and further genetic analysis include the two FHB QTLs on chromosome 2H and one on 6H, which were also associated with low DON and later heading date in multiple environments. This study provides a starting point for manipulating Zhedar 2-derived resistance by MAS in barley to develop cultivars that will show effective resistance under disease pressure.