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Title: IDENTIFICATION AND CHARACTERIZATION OF LORF11, A GENE UNIQUE TO THE THREE SEROTYPES OF MAREK'S DISEASE VIRUS

Author
item Lee, Lucy
item CUI, XIAOPING - MICHIGAN STATE UNIVERSITY
item REDDY, SANJAY - UNIV OF TEXAS A&M
item Silva, Robert

Submitted to: World Veterinary Poultry Association Congress Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 7/19/2003
Publication Date: 7/31/2003
Citation: Lee, L.F., Cui, X., Reddy, S., Silva, R.F. 2003. Identification and characterization of lorf11, a gene unique to the three serotypes of marek's disease virus. World Veterinary Poultry Association Congress Proceedings. p. 145.

Interpretive Summary:

Technical Abstract: We have recently completed DNA sequencing the unique long region of the GA strain of Marek's disease virus (MDV) and revealed a large open reading frame (LORF11) comprising 2711 nucleotides in length and potentially encoding a protein of 903 amino acids. DNA sequence comparison between all three MDV serotypes revealed all three serotypes contained LORF11 homologs. Because of its uniqueness and the size of the gene, we are interested in studying its biological function. Using a synthetic peptide, we developed antiserum against LORF11 and found the gene product was expressed in all three serotypes of MDV. Using a series of overlapping cosmids that spanned the MDV genome, we constructed deletion mutants in order to evaluate its biological function.