Author
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Min, Wongi |
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Lillehoj, Hyun |
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Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 7/1/2003 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: In this study, we isolated and initially characterized cDNAs encoding IL-16 and IL-17 using a chicken intestinal library. IL-16 and IL-17 are proinflammatory cytokines produced by activated lymphocyte cells. Chicken Il-16 gene contained the entire open reading frame (ORF) of pro-IL-16 and encoded the mature IL-16 protein which consists of 607 amino acids. Chicken IL-16 showed 86% sequence homology to duck pro-IL-16 and 49-52% to various mammalian IL-16s. By Northern blot analysis, IL-16 transcripts showed a restricted expression to the lymphoid tissues. Recombinant chicken IL-16 consisting of 149 C-terminal amino acids of pro-IL-16 was biologically active when expressed in COS-7 cells and showed chemoattractant property for lymphocytes. IL-17 contained a 507 bp open reading frame predicted to encode a protein of 169 amino acids with a molecular mass of 18.9 kDa. Chicken IL-17 shared 37%-46% amino acid sequence identity with mammalian IL-17 and homologous to the ORF 13 of Herpesvirus saimiri. By Northen blot analysis, IL-17 transcripts were identified in a reticuloendotheliosis virus-transformed chicken lymphoblast cell line (CU205) and Con A-stimulated splenic lymphocytes, but not Cu91, RP9, RP13 cell lines or kidney, bursa, heart, spleen, cecal tonsils and thymus. Conditioned medium from COS-7 cells transfected with chicken IL-17 cDNA induced the production of IL-6 by embryonic fibroblasts. Key words: Chicken, Inteleukin-16, Interleukin-17 |
