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Title: IDENTIFICATION OF FIVE OUTER MEMBRANE-ASSOCIATED PROTEINS OF CROSS-PROTECTIVE FACTOR PROTEINS OF PASTEURELLA MULTOCIDA

Author
item Tabatabai, Louisa
item Zehr, Emilie

Submitted to: Infection and Immunity
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/23/2003
Publication Date: 2/1/2004
Citation: TABATABAI, L.B., ZEHR, E.S. IDENTIFICATION OF FIVE OUTER MEMBRANE-ASSOCIATED PROTEINS OF CROSS-PROTECTIVE FACTOR PROTEINS OF PASTEURELLA MULTOCIDA. INFECTION AND IMMUNITY. 2004. 72:1195-1198.

Interpretive Summary: Commercial bacterins for the control of fowl cholera are comprised of P. multocida serotypes A:1, A:3, and A:4, which are the serotypes that are most often associated with outbreaks in poultry flocks. A membrane-associated protein fraction (cross-protective factor, CPF) containing 11 major proteins was reported to be responsible for the observed protection against challenge with a virulent strain of P. multocida. A 39-kDa protein was believed to be one of the proteins conferring protection. We have identified the 39-kDa protein as the lipoprotein B. This protein belongs to a family of bacterial lipoproteins that may be involved in bacterial virulence. Four additional proteins were identified, including a high molecular weight putative virulence factor, capsular biosynthetic protein, a porphyrin biosynthetic protein, and a protein involved in cell membrane biogenesis.

Technical Abstract: Commercial bacterins for the control of fowl cholera are comprised of P. multocida serotypes A:1, A:3, and A:4, which are the serotypes that are most often associated with outbreaks in poultry flocks. A membrane-associated protein fraction (cross-protective factor, CPF) containing 11 major proteins was reported to be responsible for the observed protection against challenge with a virulent strain of P. multocida. A 39-kDa protein was believed to be one of the proteins conferring protection. By using peptide mass fingerprinting and MALDI-TOF mass spectrometry, we have identified two lipoproteins (lipoprotein B, PlpB, Mr of 30,233 and an unknown lipoprotein, Mr of 37,374 kDa) and three membrane-associated proteins (lipase A, LipA/PhyA, Mr of 79,738; putative virulence factor, Mr of 117,437; and porphyrin biosynthetic protein, HemY, Mr of 47,773). Our data suggested that the 39-kDa protein was identical to PlpB. Protein identity was confirmed by N-terminal sequence determination of two HPLC-purified tryptic peptides. PlpB belongs to the pfam03180 family of bacterial lipoproteins that may be involved with bacterial virulence.