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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #152546
Title: DISSECTING THE MAIZE GENOME USING A RADIATION HYBRID APPROACH

Author
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA
item Rines, Howard
item OKAGAKI, R - UNIVERSITY OF MINNESOTA
item KYNAST, R - UNIVERSITY OF MINNESOTA
item ODLAND, W - UNIVERSITY OF MINNESOTA
item STEC, A - UNIVERSITY OF MINNESOTA
item JACOBS, M - UNIVERSITY OF MINNESOTA
item GRANATH, S - UNIVERSITY OF MINNESOTA

Submitted to: From the Green Revolution to the Gene Revolution Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 5/28/2003
Publication Date: 5/28/2003
Citation: Phillips, R.L., Rines, H.W., Okagaki, R., Kynast, R., Odland, W., Stec, A., Jacobs, M., Granath, S. 2003. Dissecting the maize genome using a radiation hybrid approach [abstract]. From The green Revolution to the Gene Revolution Symposium. p. 37.

Interpretive Summary:

Technical Abstract: The crossing of oat with maize by application of maize pollen to oat florets results in about one-third of the plants recovered by embryo rescue having the haploid number of oat chromosomes and one or more maize chromosomes. We have now derived all ten possible oat-maize addition lines where an individual maize chromosome is present together with a complete set of oat chromosomes. Disomic versions of all addition lines except for chromosome 10 are available. Phenotypes are described that are associated with the addition lines and in some instances how these phenotypes relate to the expression of maize genes in an alien background. Monosomic oat-maize addition lines are irradiated to obtain derivatives that either have a diminutive version of the maize chromosome or a translocation to the oat genome. In either case, stocks called Radiation Hybrids (RHs) are derived that have one or more deficiencies of the maize chromosome. These lines allow DNA sequences to be mapped to a sub-chromosome segment of the maize chromosome. Our goal is to have a set of RHs for each chromosome that will allow the mapping of DNA sequences to a 5 Mb region. This report summarizes the status of RH production for each individual chromosome and illustrates the mapping of ESTs and the relationship to the two sub-genome nature of the maize genome.