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Title: DEVELOPMENT OF ANIMAL CELL LINES FOR USE IN GENOMIC STUDIES

Author
item Chitko-Mckown, Carol
item Freking, Bradley - Brad
item Macneil, Michael
item Smith, Timothy - Tim

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/6/2003
Publication Date: 11/9/2003
Citation: CHITKO MCKOWN, C.G., FREKING, B.A., MACNEIL, M.D., SMITH, T.P. DEVELOPMENT OF ANIMAL CELL LINES FOR USE IN GENOMIC STUDIES. RESEARCH WORKERS IN ANIMAL DISEASES CONFERENCE PROCEEDINGS. 2003. ABSTRACT NO. 90P.

Interpretive Summary:

Technical Abstract: Performing genomic studies on specific animals requires that abundant quantities of DNA from these animals be available. If the animal is alive and readily accessible, blood or other tissues can be collected and used for the isolation of genetic material. However, if the animal is deceased, or not easily accessible, cell lines developed from these animals can be stored indefinitely, can be shipped to distant locations, and can provide a relatively unlimited source of DNA. In order to develop cell lines, we obtained tissues from a Texel ram whose DNA was used to construct a BAC library, as well as skin biopsy samples from a cow whose DNA was being used to sequence the bovine genome. The tissues were subjected to homogenization and treatment with trypsin in order to produce cell suspensions. After a several-week period of culture, foci of cell growth were apparent. The cell cultures were expanded and aliquots frozen in liquid nitrogen for storage. At this time, we have successfully developed fibroblast and endothelial cells lines from the Texel ram and a fibroblast cell line from the bovine genome project cow. These lines will be made publicly available to research centers interested in utilizing them for additional genetic studies.