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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #153978
Title: INDUCED MATURATION AND SPAWNING: OPPORTUNITIES AND APPLICATIONS FOR RESEARCH ON OOGENESIS

Author
item SULLIVAN, CRIAG - N CAROLINA STATE U
item HIRAMATSU, NAOSHI - N CAROLINA STATE U
item KENNEDY, ALANNA - N CAROLINA STATE U
item CLARK, ROBERT - N CAROLINA STATE U
item Weber, Gregory - Greg
item MATSUBARA, TAKAHIRO - HNFRI, HOKKAIDO
item HARA, AKIHIKO - GSFS HOKKAIDO U

Submitted to: Fish Physiology and Biochemistry Journal
Publication Type: Review Article
Publication Acceptance Date: 3/23/2003
Publication Date: 1/8/2004
Citation: Sullivan, C., Hiramatsu, N., Kennedy, A.M., Clark, R.W., Weber, G.M., Matsubara, T., Hara, A. 2004. Induced maturation and spawning: opportunities and applications for research on oogenesis. Fish Physiology and Biochemistry Journal.

Interpretive Summary:

Technical Abstract: Broodstock management requires the ability to detect and regulate oocyte growth, acquisition of maturational competence, maturation of oocytes, and onset of ovarian atresia. Our research on temperate basses (genus Morone) has supported development of these capabilities. These investigations have revealed that accumulation of neutral lipid droplets and deposition of vitellogenin-derived yolk proteins in growing oocytes are independent processes with different sensitivities to changing day length and water temperature. In these fishes, completion of oocyte growth is marked by disappearance of vitellogenin from ovarian biopsy samples. Comptence of females for induced spawning is predicted by the ability of biopsied follicles to initiate oocyte meiosis in vitro in response to insulin-like growth factor I. Cytoplasmic maturation of the oocyte is triggered by the maturation-inducing steroid hormone and can be monitored by evaluating degradation of the yolk proteins. Onset of ovarian atresia is indicated by the appearance of edema in the granulose cell layer of biopsied follicles, and can be delayed for months by holding gravid females at abnormally low temperature ('cold banking'). These novel findings hold promise for application to other farmed fishes.