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Title: PHENOLIC ACID ESTERASES, CODING SEQUENCES, AND METHODS

Author
item Li, Xin Liang
item LJUNGDAHL, LARS - ATHENS, GA
item AZAIN, MICHAEL - WATKINSVILLE, GA
item DAVIES, EDWARD - ATHENS, GA
item SHAH, ASHIT - ATHENS, GA
item BLUM, DAVID - SAN DIEGO, CA
item KATAEVA, IRINA - ATHENS, GA

Submitted to: Patent Application
Publication Type: Popular Publication
Publication Acceptance Date: 6/21/2000
Publication Date: 8/5/2003
Citation: Li, X., Ljungdahl, L.G., Azain, M.J., Davies, E.T., Shah, A.K., Blum, D.L., Kataeva, I. 2003. Phenolic acid esterases, coding sequences, and methods. U.S. Patent 6,602,700.

Interpretive Summary:

Technical Abstract: Described herein are four phenolic acid esterases, three of which correspond to domains of previously unknown function within bacterial xylanases, from XynY and XynZ of Clostridium thermocellum and from a feruloyl esterase of Ruminococcus. The fourth specifically exemplified phenolic acid esterase is a protein encoded within the genome of Orpinomyces PC-2. The amino acids of these polypeptides and nucleotide sequences encoding them are provided. Recombinant host cells, expression vectors and methods for the recombinant production of phenolic acid esterases are also provided. Further provided are methods for improving nutrient availablility and ferulic acid availability when food or feed, or other material is treated with a phenolic acid esterase, desirably in combination with a xylanase.