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ARS Home » Southeast Area » Raleigh, North Carolina » Soybean and Nitrogen Fixation Research » Research » Publications at this Location » Publication #154548
Title: QUANTITATIVE TRAIT LOCI FOR BETA-CONGLYCININ (7S) AND GLYCININ (11S) IN SOYBEAN STORAGE PROTEIN

Author
item PANTHEE, D - UNIV OF TENNESSEE
item Kwanyuen, Prachuab
item SAMS, C - UNIV OF TENNESSEE
item WEST, D - UNIV OF TENNESSEE
item SAXTON, A - UNIV OF TENNESSEE
item PANTALONE, V - UNIV OF TENNESSEE

Submitted to: Journal of the American Oil Chemists' Society
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/15/2004
Publication Date: 11/15/2004
Citation: Panthee, D.R., Kwanyuen, P., Sams, C.E., West, D.R., Saxton, A.M., Pantalone, V.R. 2004. Quantitative trait loci for beta-conglycinin and glycinin in soybean storage proteins. Journal of American Oil Chemists' Society. Vol. 81, no. 11, 1005-1012.

Interpretive Summary: A major limitation in both nutritional and functional qualities of storage proteins in soybean seed is the lack of sulfur-containing amino acids, methionine and cysteine. The objective of this study was to map quantitative trait loci (QTL) that govern these proteins in soybean. One hundred and four recombinant inbred lines from a population developed from a cross of two high-protein soybeans were used for the analyses of their protein composition. A significant difference among genotypes was found for the two major storage proteins, beta'conglycinin and glycinin. A total of 86 polymorphic simple sequence repeat of molecular genetic markers were also screened in DNA from those progenies. The results revealed that QTL were distributed in five different linkage groups that govern these two storage proteins. Phenotypic variation explained by individual QTL ranged from 5.4 to 11.8%. These QTL may provide useful marker assisted selection opportunities for improvement of nutritional quality in soybean.

Technical Abstract: Glycinin (11S) and beta-conglycinin (7S) are important seed storage proteins in soybean (Glycine max L.). A major limitation of soybean seed storage proteins is their lack of the sulfur-containing amino acids, methionine and cysteine. Glycinin contains significantly higher S-containing amino acids than beta-conglycinin. The objective of this study was to map quantitative trait loci (QTL) governing 7S and 11S seed storage proteins in soybean. To achieve this objective, 104 F6:8 recombinant inbred lines (RIL) from a population developed from a cross of N87-984-16 x TN93-99 were used. Storage proteins were extracted from all RIL and separated in 10-20% linear gradient polyacrylamide gels. Dried gels were scanned for individual subunits of storage protein with a Densitometer equipped with a He-Ne laser light source. The data were converted to concentration for each subunit component, and analyzed using SAS software. A significant (P<0.05) difference among genotypes was found for glycinin and beta-conglycinin. A total of 86 polymorphic simple sequence repeat (SSR) molecular genetic markers were screened in DNA from progenies. Single factor ANOVA revealed that QTL were distributed in five different linkage groups namely D1a, D1b, L, M and N governing 7S and 11S in soybean seed. Phenotypic variation explained by individual QTL ranged from 5.4 to 11.8%. These QTL may provide useful marker assisted selection opportunities for improvement of nutritional quality in soybean.