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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Food Animal Metabolism Research » Research » Publications at this Location » Publication #155097
Title: ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR THE NEONICOTINOID INSECTICIDES.

Author
item KIM, HEE-JOO - UNIV OF HAWAII
item Shelver, Weilin
item KEUM, YOUNG-SOO - UNIV OF HAWAII
item HWANG, EUL-CHUL - UNIV OF HAWAII
item LI, QING - UNIV OF HAWAII

Submitted to: Korean Journal of Agricultural Chemistry and Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/24/2003
Publication Date: 12/1/2003
Citation: Kim, H., Shelver, W.L., Keum, Y., Hwang, E., Li, Q.X. 2003. Enzyme-linked immunosorbent assays for the neonicotinoid insecticides. Korean Journal of Agricultural Chemistry and Biotechnology 46(4):133-136.

Interpretive Summary: The neonicotinoids are a new class of insecticides utilized to control sucking insects such as rice hoppers, aphids, thrips, whiteflies, termites, turf insects, soil insects, and beetles. They can be used as a crop and structural pest insecticide, a seed treatment, and a flea-control treatment. Enzyme-linked immunosorbent assays (ELISAs) are sensitive, selective, and capable of cost effective and high throughput, which make them ideal for the determination of pesticides in a myriad of applications where these properties are required. ELISAs developed by different laboratories for the neonicotinoid insecticides can be an alternative method to analyze these insecticides in environmental samples and can overcome some of the shortcomings of conventional instrumental analysis. Neonicotinoids are the fastest selling insecticide class in the world and are expected to be extensively used. As such, residues may present environmental and food safety problems. Therefore, a rapid assay, such as ELISA, is needed. This mini review summarizes currently available ELISAs for the neonicotinoid insecticides imidacloprid, acetamiprid and thiamethoxam.

Technical Abstract: The neonicotinoids are a new class of insecticides acting as agonists at the postsynaptic nicotinic acetylcholine receptors. Enzyme-linked immunosorbent assays (ELISAs) are sensitive, selective, and capable of cost effective and high throughput, which make them ideal for the determination of pesticides in a myriad of applications where these properties are required. ELISAs developed by different laboratories for the neonicotinoid insecticides can be an alternative method to analyze these insecticides in environmental samples and can overcome some of the shortcomings of conventional instrumental analysis. This mini review summarizes ELISAs currently available for the neonicotinoid insecticides imidacloprid, acetamiprid, and thiamethoxam.