Author
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Veremis, John |
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Lingle, Sarah |
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Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only Publication Acceptance Date: 10/31/2003 Publication Date: 1/10/2004 Citation: Veremis, J.C., Lingle, S.E. 2004. Genotypic diversity in the promoter region and introns of sucrose synthase-2 in the genus Saccharum [abstract]. In: Plant and Animal Genome XII Final Abstracts Guide. Plant and Animal Genome XII Conference, January 10-14, 2004, San Diego, California. p. 33. Interpretive Summary: Technical Abstract: Sucrose synthase (SuSy) is an important sucrose metabolism enzyme in sugarcane. Variation in activity of SuSy may be related to sucrose accumulation. Northern analysis showed that the sucrose synthase gene Sus2 (homologous to maize Sh1) is more highly expressed in sucrose-storing sugarcane genotypes than low-sucrose wild genotypes. We designed PCR primers to amplify the promoter region and 14 introns of the Sus2 gene in a number of Saccharum species and hybrids. There were polymorphisms in the promoter region and in six introns. These polymorphisms were shown to be inherited by the progeny of interspecific crosses. With the above variation we will determine a core set of trait-specific DNA markers most suitable for differentiating of Saccharum species hybrids. While it is not yet known if any of the polymorphisms influence transcription of the gene, these differences may be useful for the selection of the highly expressed form of the gene from our interspecific progenies. Marker-assisted selection of the Sus2 gene will allow us to systematically backcross and recombine progeny in a manner that will fix different alleles to test the hypothesis that variations in the Sus2 gene contribute to sucrose concentration in sugarcane. |
