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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #156238
Title: IDENTIFICATION OF ANONYMOUS MICROSATELLITE MARKERS FOR MAPPING IN RAINBOW TROUT

Author
item COULIBALY, I - WEST VIRGINIA U
item Rexroad, Caird

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/10/2004
Publication Date: 1/10/2004
Citation: Coulibaly, I., Rexroad III, C.E. 2004. Identification of anonymous microsatellite markers for mapping in rainbow trout. Plant and Animal Genome Abstracts. Abstract P718. p.250.

Interpretive Summary:

Technical Abstract: The genetic improvement of species for aquaculture production efficiency is enhanced by the use of molecular genetic technologies. The development of genetic maps is critical to the molecular genetic approach and requires the development of large numbers of polymorphic genetic markers. NCCCWA has chosen microsatellite markers for the construction of a genetic linkage map for rainbow trout (Oncorhynchus mykiss) due to their co-dominant inheritance and usefulness for analyses between families. The international research community has previously characterized and mapped a large number of microsatellite markers in rainbow trout, the goal of this project is to generate a new set of anonymous markers that will result in genetic maps with even greater resolution. Microsatellite repeat sequences were identified by single pass sequencing a total of 2246 clones from ATG, CA and GA microsatellite repeat-enriched libraries. The sequences were quality (Phred 20) and vector trimmed, screened for repeat motifs, and analyzed for redundancy with previously characterized markers. As a result, 726 new unique sequences were identified of which 261 originated from the ATG library, 283 from the CA library and 182 from the GA library. Most sequences from the ATG and GA libraries contain repeats corresponding to the library enrichment motif. Only a quarter of the sequences from the CA library actually contain CA repeats. PCR primer design, optimization, and genotyping on the NCCCWA reference families are underway for these markers. The goal of this project is to develop at least 200 additional microsatellite markers for genetic mapping in rainbow trout.