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ARS Home » Southeast Area » Little Rock, Arkansas » Microbiome and Metabolism Research Unit » Research » Publications at this Location » Publication #156894
Title: FEEDING DIETS CONTAINING SOY PROTEIN ISOLATE (SPI) OR ISOFLAVONES AT WEANING ALTERS CYP4A AND CYP2C EXPRESSION IN RAT KIDNEY TO FAVOR PRODUCTION OF ANTI-HYPERTENSIVE ARACHIDONIC ACID PRODUCTS.

Author
item RONIS, MARTIN - ACNC/UAMS
item DAHL, CHRISTOPHER - ACNC
item BADGER, THOMAS - ACNC/UAMS

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/10/2004
Publication Date: 3/29/2004
Citation: Ronis, M.J., Dahl, C., Badger, T.M. 2004. Feeding diets containing soy protein isolate (spi) or isoflavones at weaning alters cyp4a and cyp2c expression in rat kidney to favor production of anti-hypertensive arachidonic acid products. The FASEB Journal. v. 18(4):102.4.

Interpretive Summary: Soybeans are reported to have several health benefits and soy protein isolate (SPI) has been granted a health claim for its ability to help control cholesterol in humans and thus help prevent cardiovascular disease. SPI may be able to reduce blood pressure by altering the metabolism of arachidonic acid (AA). AA is converted to 20-hydroxyeicosatetraenoic acid which increases blood pressure by a proteins known as CYP4As and then converted to a compound that reduces blood pressure known as epoxyeicosatrienoic acids by proteins known as CYP2Cs. Our data demonstrate that SPI may be able to reduce blood pressure or help prevent high blood pressure from developing by increasing the CYP2C11-type proteins that lead to production of anti-hypertensive epoxyeicosatrienoic acids.

Technical Abstract: Soy has many biochemical effects and may have anti-hypertensive properties. Increases in expression of cytochrome P450 enzymes in gene family 4A and decreases in family 2C in rat kidney have been associated with the development of hypertension as the result of a shift in arachidonic acid metabolism away from formation of pro-hypertensive 20-hydroxyeicosatetraenoic acid by CYP4As to anti-hypertensive epoxyeicosatrienoic acids by CYP2Cs. Male or female pups (N = 8-10) were weaned from rat dams fed semi-purifed diets made with casein as the sole protein source onto diets containing either casein (CAS) or soy protein isolate (SPI+); SPI stripped of isoflavones (SPI-) or CAS plus the soy isoflavones genistein or daidzein at 1 mg/kg until sacrifice at post-natal d 34. Feeding SPI+ or SPI- reduced CYP4A1 mRNA expression in the kidney of both sexes. In addition feeding either soy diet reduced expression of male predominant CYP4A2 in male kidney, but had little effect in females. In contrast, while CYP4As were also reduced by feeding daidzein, genistein diets had no effect. Soy diets also reduced renal expression of male specific CYP2C11 mRNA but had no effect on expression of CYP2C23. In contrast, feeding genistein increased both CYP2C11 and CYP2C23 mRNA (p < 0.05). These data suggest feeding soy or isofavones may be able to reduce high blood pressure though altered kidney metabolism of arachidonic acid.