Author
YAMADA, KAYOKO - ARS-UCB PLNT GENE EXP CTR | |
LIM, JUN - SALK INSTITUTE BIOL | |
DALE, JOSEPH - ARS-UCB PLNT GENE EXP CTR | |
CHEN, HAUMING - SALK INST/UNIV PA | |
SHINN, PAUL - SALK INST/UNIV PA | |
PALM, CURTIS - STANFORD GENOME TECH | |
WU, HANK - ARS-UCB PLNT GENE EXP CTR | |
PHAM, PAUL - ARS-UCB PLNT GENE EXP CTR | |
LIU, SHIRLEY - ARS-UCB PLNT GENE EXP CTR | |
SAKANO, HITOMI - ARS-UCB PLNT GENE EXP CTR | |
YU, GUIXIA - ARS-UCB PLNT GENE EXP CTR | |
QUACH, HONG - ARS-UCB PLNT GENE EXP CTR | |
AKIYAMA, KENJI - RIKEN TSUKUBA JAPAN | |
ARAKAWA, TAKAHIRO - RIKEN KANAGAWA JAPAN | |
CARNINCI, PEIRO - RIKEN WAKO/KANAGAWA | |
SEKI, MOTOAKI - RIKEN TSUKUBA JAPAN | |
DAVIS, RONALD - STANFORD UNIV | |
Theologis, Athanasios | |
ECKER, JOSEPH - SALK INST/UNIV PA |
Submitted to: Science Magazine
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 10/21/2003 Publication Date: 10/31/2003 Citation: YAMADA, K., LIM, J., DALE, J., CHEN, H., SHINN, P., PALM, C.J., WU, H.C., PHAM, P., LIU, S.X., SAKANO, H., YU, G., QUACH, H.L., AKIYAMA, K., ARAKAWA, T., CARNINCI, P., SEKI, M., DAVIS, R.W., THEOLOGIS, A., ECKER, J.R. EMPIRICAL ANALYSIS OF TRANSCIRPTIONAL ACTIVITY IN THE ARABIDOPSIS GENOME. SCIENCE MAGAZINE. 2003. 302(5646):842-6. Interpretive Summary: Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome. Technical Abstract: Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome. |