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Title: ADVANCED BACKCROSS QTL ANALYSIS IN A MATING BETWEEN GLYCINE MAX AND GLYCINE SOJA

Author
item CHAKY, J - UNIVERSITY OF NEBRASKA
item SPECHT, J - UNIVERSITY OF NEBRASKA
item Cregan, Perry

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/5/2003
Publication Date: 10/5/2003
Citation: Chaky, J.M., Specht, J.E., Cregan, P.B. 2003. Advanced backcross qtl analysis in a mating between glycine max and glycine soja [abstract]. Plant and Animal Genome Abstracts. P545.

Interpretive Summary:

Technical Abstract: The advanced backcross (AB) method is a means of reducing the number of donor parent alleles present in any given backcross inbred line (BIL). With fewer donor alleles present, deleterious alleles can be readily exposed; conversely, favorable donor alleles at quantitative trait loci (QTLs) can be more easily recognized. To evaluate the AB method of QTL detection in soybean, a population of 296 BC2F4.6 BILs was generated from a mating between Glycine max (Dunbar) and donor parent Glycine soja (PI 326582A). The 296 BILs have now been genotyped at 157 simple sequence repeat (SSR) marker loci and four classical marker loci, creating a 1201.2 centimorgan (cM) genetic map of 39 linkage groups (LGs) that, on the basis of SSR map position, were aligned with the 20 published soybean LGs. There were some positive transgressive BIL segregants for seed yield, but none were significantly better than Dunbar. In the QTL analyses, a significant seed yield QTL was detected on LG-A2, flanked by SSR marker Satt315 and classical marker I; however, the additive effect of the PI 326.582A allele was a seed yield reduction of '226 kg ha-1. No other significant yield QTLs were detected. In fact, all introgressed genomic segments of this G. soja parent had negative (though not statistically significant) effects on yield, with the exception of some LG-C2 segments that had no additive effect. Two QTLs that affected seed protein and oil were mapped to LG-I and LG-E. The introgressed PI 326.582A alleles enhanced seed protein by 1.4 (LG-I) and by 0.7 (LG-E) percentage points, but pleiotropically reduced oil by 0.5 (LG-I) and 0.4 (LG-E) percentage points. Although a majority of the BILs were lower yielding than Dunbar, 27 BILs yielded about the same as the 24 Dunbar check entries. This suggested that the Dunbar genome can, without deleterious effect, tolerate introgression of some G. soja genome. However, many of these 27 BILs were later maturing and taller, which may have confounded the yield comparison.