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ARS Home » Southeast Area » Fayetteville, Arkansas » Poultry Production and Product Safety Research » Research » Publications at this Location » Publication #158360
Title: EVALUATION OF PROBIOTIC CULTURES ON SALMONELLA PREVALENCE IN COMMERCIAL TURKEY HOUSES.

Author
item VINCENTE, J - UNIV OF ARKANSAS
item NAVA, G - UNIV OF ARKANSAS
item TORRES, A - UNIV OF ARKANSAS
item JARQUIN, R - UNIV OF ARKANSAS
item SARTOR, C - UNIV OF ARKANSAS
item WOLFENDEN, A - UNIV OF ARKANSAS
item HIGGINS, S - UNIV OF ARKANSAS
item BIELKE, L - UNIV OF ARKANSAS
item TELLEZ, G - UNIV OF ARKANSAS
item Donoghue, Ann - Annie

Submitted to: Southern Poultry Science Society Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2003
Publication Date: 9/29/2004
Citation: Vincente, J.L., Nava, G.M., Torres, A., Jarquin, R., Sartor, C.D., Wolfenden, A., Higgins, S., Bielke, L., Tellez, G., Donoghue, A.M. 2004. Evaluation of probiotic cultures on salmonella prevalence in commercial turkey houses [abstract]. Poultry Science. 83(Suppl 1):1770-1771.

Interpretive Summary:

Technical Abstract: The efficacy of a 9-bacteria (7 enterobacteriaceae and 2 lactic acid bacteria) probiotic culture (P1) and two lactic acid bacteria isolates alone (P2), with or without a commercial organic acid (OA), were evaluated for ability to reduce recoverable environmental Salmonella in commercial turkey flocks 2 weeks prior to processing. Salmonella positive flocks were identified 3 to 4 weeks prior to processing by standard drag swab sampling of each house with subsequent enrichment and selective plating. Eight drag swab assemblies were utilized per house, and houses with Salmonella recovery from > 6 of 8 drag swabs were selected as candidates for treatment. Two weeks after treatment (prior to live haul), similar drag swab assemblies were utilized and Salmonella recovery incidence was compared following enrichment on novobiocin-containing BGA plates with serological confirmation of suspect colonies. In the first trial, 6 Salmonella-positive houses were selected to evaluate four treatments, a) P1-100X, b) OA+P1-100X, c) OA+P1, and d) OA+P2. A stock solution of OA (Performax) was prepared (0.95 L OA/7.8 L water) and added to the drinking water for 8h (128x dilution). After cleaning the water lines by flushing with tap water, the cultures were administered in the drinking water at a final concentration of 1.0 x 10 6 cfu/ml for three consecutive days. Drag swab samples were taken two weeks after treatment, prior to live haul. A significant reduction (P<0.01) of Salmonella recovery was observed in all treated houses two weeks after treatment (a: 90%, b: 100%, c: 100%, and d: 86% reduction). In the second trial, 22 Salmonella-positive houses were selected to evaluate six treatments, a) Control, b) OA, c) P1, d) P2, e) OA+P1, and f) OA+P2. Two weeks after treatment, the recovery of Salmonella was significantly reduced (P<0.001) in houses treated with P1 or P2 in combination with OA, when compared to control houses (87 and 75% reductions, respectively). These studies suggest that the administration of selected probiotics in combination with OA can reduce the recovery of environmental Salmonella in turkeys houses prior to processing.