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ARS Home » Pacific West Area » Wapato, Washington » Temperate Tree Fruit and Vegetable Research » Research » Publications at this Location » Publication #159955
Title: DNA-BASED DIAGNOSTICS FOR LEPIDOPTERAN PESTS OF POME FRUITS

Author
item BARCENAS, NINA - WA ST UNIV,DEPT ENTOMOLOG
item Unruh, Thomas
item Neven, Lisa

Submitted to: Western Orchard Pest and Disease Management Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2003
Publication Date: 1/14/2004
Citation: Barcenas, N., Unruh, T.R., Neven, L.G. 2004. DNA-based diagnostics for Lepidopteran pests of pome fruits. Western Orchard Pest and Disease Management Conference. p.35.

Interpretive Summary: Abstract only; please refer to technical abstract section

Technical Abstract: CO-1 mitochondrial DNA from the 4 tortricids which feed internally in apples and pears in the USA was sequenced and compared. Primer sets were designed that selectively amplify each species and protocols were developed for a real-time PCR instrument and for traditional PCR followed by agarose electrophoresis. Cydia pomonella and the 3 Grapholita species (G. molesta, G. packardi, G. prunivora) are readily separated. The technique is robust for geographically disparate populations of each species. Mexico is now using the method to confirm identifications of larvae found in intercepted fruit. A possible new species of Grapholita, near packardi was seen attacking rose hips in Washington.