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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #160542
Title: GENETIC MARKERS ASSOCIATED WITH RESISTANCE TO INFECTIOUS HEMATOPOIETIC NECROSIS IN RAINBOW AND STEELHEAD TROUT (ONCORHYNCHUS MYKISS) BACKCROSSES

Author
item Rodriguez, Maria
item LAPATRA, SCOTT - CLEARSPRINGS CORP.
item WILLIAMS, SCOTT - CLEARSPRINGS CORP.
item FAMULA, THOMAS - UNIVERSITY OF CAL. DAVIS
item MAY, BERNIE - UNIVERSITY OF CAL. DAVIS

Submitted to: Aquaculture
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/30/2004
Publication Date: N/A
Citation: N/A

Interpretive Summary: Infectious hematopoietic necrosis (IHN) is a highly infectious disease that causes significant economic losses to the salmonid industry. There is great interest in developing a rainbow trout lineage that possesses improved resistance for this disease. To accomplish this goal, it will be necessary to identify genes that affect this trait. The use of genetic markers to conduct linkage studies is a strategy for identifying these important genes. This paper reports the finding of several markers putatively associated with IHN resistance in rainbow trout and steelhead trout backcrosses.

Technical Abstract: Backcrosses of rainbow trout and steelhead (Oncorhynchus mykiss) were used to construct a linkage map and to identify associations between molecular markers and quantitative trait loci (QTL) determining resistance to infectious hematopoietic necrosis virus (IHNV). The sire map covers 1019cM, and it is composed of 257 molecular markers (185 AFLPs, and 72 microsatellites) distributed over 28 linkage groups. The dam map consists of 236 markers (164 AFLPs and 72 microsatellites), with a total of 45 linkage groups, covering 2041 cM. Sixty one new microsatellite markers were added to the existing rainbow trout linkage maps. Seven AFLPs and six microsatellites in the dam map were associated with IHNV resistance. Eighteen AFLPs and six microsatellites associated with IHNV resistance were located in linkage groups 11, 20 and 25 in the sire map. Microsatellite markers mapped to the three linkage groups were used to screen nine other families, and QTL-marker associations were repeated for four of the markers in at least one of the families. Further marker mapping is required to increase resolution and facilitate positional cloning of QTLs affecting IHNV resistance.