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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #160553
Title: COMPLETE NUCLEOTIDE SEQUENCE OF A STRAWBERRY ISOLATE OF BEET PSEUDOYELLOWS VIRUS

Author
item TZANATEKIS, I - OREGON STATE UNIVERSITY
item Martin, Robert

Submitted to: Virus Genes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/1/2003
Publication Date: 4/1/2004
Citation: Tzanatekis, I.E., Martin, R.R. Complete nucleotide sequence of a strawberry isolate of beet pseudoyellows virus. Virus Genes. 2004. v28 p. 239-246.

Interpretive Summary: We have identified and characterized Beet pseudoyellows viurs as a second virus capable of causing strawberry pallidosis disease in strawberry. Obtaining the sequence of this virus allowed us to develop a single laboratory test to detect both viruses that cause strawberry pallidosis disease. This test is now being applied in certification programs and will result in the production of higher quality plants for the strawberry industry. The test developed in this work has been used to identify this virus in other crops, including blackberry and pumpkin.

Technical Abstract: In our effort to identify the causal agent(s) of strawberry pallidosis, a disease first identified in 1957, we found a single plant that was pallidosis positive by grafting that did not give any amplicons after RT-PCR using primer sets representing multiple regions of Strawberry pallidosis associated virus (SPaV) genome nor did it give any positive reactions using antibodies derived against the recombinant coat protein gene (CP) of SPaV. DsRNA extracted from this plant showed a similar pattern to that of SPaV indicating that another crinivirus may infect strawberry. We cloned parts of the unknown virus and sequenced multiple clones that corresponded to the already published sequence of Beet pseudo yellows virus (BPYV) heat shock 70 homolog gene (HSP70h), the trademark of the viruses belonging to the Closteroviridae family. We have acquired the complete nucleotide sequence of a strawberry isolate of BPYV. This isolate has several features that do not match Cucumber yellows virus (CuYV), a strain of the virus that has been sequenced recently, including an ORF not found in CuYV. The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers AY 330918 and AY 330919.