Author
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Evens, Terence |
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Niedz, Randall |
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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 3/9/2004 Publication Date: 5/21/2004 Citation: Evens, T.J., Niedz, R.P. 2004. ARS-media: a new public-domain software package for the design and evaluation of plant tissue-culture media. Meeting Abstract. Interpretive Summary: Technical Abstract: The success of plant tissue culture is highly influenced by the nature of the culture medium used. In general, media formulas are complex mixtures of inorganic salts, vitamins, amino acids, sugars, undefined supplements and buffers, and are modifications of recipes that were empirically derived. Media selection depends on the species of plant, the kind of culture and/or the cultural stage being undertaken. Consequently, in order to choose or formulate an optimal media recipe it is necessary to evaluate and/or manipulate multiple ion concentrations and ratios. This can be a daunting task if one considertes the number and variety of salts within any given recipe. Accordingly, we have developed a LabVIEW-based software package that allows users to: 1) select potential media recipes from a comprehensive database of published media recipes, 2) systematically compare multiple recipes, and 3)manipulate target recipe ion concentrations via a linear programming approach. The ARS-MEDIA program is public-domain and will be available as a stand-alone executable file after beta testing. |
