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Title: A 19MER PEPTIDE INSERTION IN THE E1 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE

Author
item Borca, Manuel
item Risatti, Guillermo
item Kutish, Gerald
item Lu, Zhiqiang
item Holinka-Patterson, Lauren
item FRENCH, RICHARD - UNIVERSITY OF CT
item SUR, JUNG-HYANG - FORMER PIADC EMPLOYEE
item Rock, Daniel

Submitted to: Positive Strand RNA Virus International Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 6/1/2004
Publication Date: 6/1/2004
Citation: Borca, M.V., Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Sur, J., Rock, D.L. 2004. A 19mer peptide insertion in the e1 glycoprotein of classical swine fever virus affects viral virulence in swine [abstract]. Positive Strand RNA Virus International Conference Proceedings. p. 109.

Interpretive Summary: Poxvirus Meeting.

Technical Abstract: Transposon linker insertion mutagenesis of a full-length infectious clone of the pathogenic classical swine fever virus (CSFV) isolate Brescia (pBIC) was used to identify genetic determinants of CSFV virulence and host range. A virus mutant, RB-C22 (RB-C22v), possessing a 19-residue tag insertion at the carboxyl end of E1 was constructed. RB-C22v and the parental virus pBIC (pBICv) exhibited similar growth characteristics on primary porcine macrophage cell cultures although RB-C22v produced significantly smaller plaques on SK6 cell cultures. In vivo, RB-C22v was markedly attenuated in swine. In contrast with pBIC infection, where mortality was 100%, all RB-C22v- infected pigs survived infection remaining clinically normal. A delay in spread to and decreased replication of RB-C22v in the tonsils were accompanied by a delay in generalization of infection, and a 10(2) to 10(7) log10 reduction in virus titers in lymphoid tissues and blood. These results indicate that a domain of E1 glycoprotein affects swine virulence