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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Grain Quality and Structure Research » Research » Publications at this Location » Publication #163206
Title: RAPID ISOLATION OF SORGHUM STARCH USING SONICATION

Author
item PARK, SEOK-HO - KANSAS STATE UNIV
item SINGH, HARMIT - KANSAS STATE UNIV
item Wilson, Jeff
item Bean, Scott

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/20/2004
Publication Date: 9/18/2004
Citation: Park, S., Singh, H., Wilson, J.D., Bean, S. 2004. Rapid isolation of sorghum starch using sonication. Abstract No. 389 in: 2004 AACC Annual Meeting Program Book. p.160. Meeting Abstract.

Interpretive Summary:

Technical Abstract: The study of cereal starches often requires the isolation of starch from the endosperm. Cereal starches have been isolated by hand-washing, wet-milling, or enzymatic methods (EM). However, these procedures are often tedious and time-consuming. Therefore, the goals of this study were to develop new, rapid and reproducible starch isolation methods using sonication. Decorticated sorghum flour was sonicated in a pH 10.0 buffer with 2% SDS and 2% reducing agent at a solvent to sample ratio of 20:1, followed by water-washing. Sonication times (2, 4, 6, 8, and 10 min) and different reducing agents (Beta-ME, DTT, and sodium meta-bisulfate) were tested. Protein content of starch was only 0.35-0.45% (db) after a 2-min sonication (using any reducing agent) and was reduced further to 0.15-0.30% (db) using longer times. The sonicated starch was comparable to starch obtained by the EM which takes several hours to complete. Starch yield (db) and protein content (db) were 64 and 0.84% for the EM starch, and 69 and 0.42% for the sonicated starch. The color of starch obtained by sonication showed a similar L (brightness) value, (93.9 vs. 93.8) and lower b (yellow) value (2.99 vs. 3.80) than the EM starch. Physiochemical properties of starches from different types of sorghum (hard vs. soft, normal vs. waxy) and the purification of starch from other cereals will be presented. Cereal starches can rapidly be isolated using this procedure.