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Title: EST ANALYSIS FOR THE STUDY OF COLD HARDINESS IN BLUEBERRY.

Author
item DHANARAJ, ANIK - IOWA STATE UNIV
item Slovin, Janet
item Rowland, Lisa

Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2004
Publication Date: 4/1/2004
Citation: Dhanaraj, A.L., Slovin, J.P., Rowland, L.J. 2004. Est analysis for the study of cold hardiness in blueberry.. BARC Poster Day.

Interpretive Summary:

Technical Abstract: To gain a better understanding of the genetics of cold hardiness in blueberry, a genomics approach based on ESTs (Expressed Sequence Tags) was used to compare genes expressed in blueberry flower buds under cold acclimating and non-acclimating conditions. Two cDNA libraries were constructed using RNA from cold acclimated and non-acclimated flower buds of the blueberry cultivar Bluecrop and about 600 ESTs generated from each of them. Sequences were compared to the publicly available sequences from Genbank, putative functions were assigned, and the cDNAs were categorized into 16 functional groups. A contig analysis was carried out as well to identify redundancies and group homologous cDNAs. cDNAs that were picked from each library the most number of times (four times or more), presumably representing highly abundant transcripts, were identified. Those that were picked more often from one library than the other library were identified as representing potentially preferentially expressed transcripts. Northern analyses were carried out using a few such cDNAs as probes to verify their preferential expression under cold acclimating or non-acclimating conditions.