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Title: ANALYSIS OF EXPRESSED SEQUENCE TAGS (EST) CDNA LIBRARY FROM EIMERIA-ACTIVATED INTESTINAL INTRA-EPITHELIAL LYMPHOCYTES

Author
item Lillehoj, Hyun
item Min, Wongi
item Ashwell, Christopher
item Van Tassell, Curtis - Curt

Submitted to: Avian Immunology Group Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/30/2004
Publication Date: 5/1/2004
Citation: Lillehoj, H.S., Min, W., Ashwell, C.M., Van Tassell, C.P. 2004. Analysis of expressed sequence tags (est) cdna library from eimeria-activated intestinal intra-epithelial lymphocytes. Avian Immunology Group Meeting, p. 60.

Interpretive Summary:

Technical Abstract: Intraepithelial lymphocytes (IELs) play a critical role in protective immune response to intestinal pathogens such as Eimeria, the etiologic agent of avian coccidiosis. Understanding local innate and acquired immune responses to enteric pathogens will lead to logical control strategies against them. In order to develop a tissue-specific cDNA microarray and high-throughput gene expression profiling tools for studying host-pathogen immunobiology in enteric diseases of poultry, normalized cDNA library was produced from Eimeria-activated intestine and high throughput sequencing of > 38,000 expressed sequence tag (EST) was conducted. A list of unique genes expressed by intestinal IELs of Eimeria-infected chickens was compiled using the expressed sequence tag (EST) strategy. The 14,409 chicken EST database consists of 1851 clusters and 7595 singletons, which revealed 9446 unique genes in the data set. Comparison of the sequence data with chicken DNA sequences in GenBank identified 125 clones encoding novel genes including two previously unidentified cytokines, interleukin (IL)-16 and IL-17. This EST library will provide a valuable resource for profiling global gene expression in normal and pathogen-infected chickens and identifying additional unique immune-related genes (Supported in part by a Cooperative State Research Service, NRI grant under agreement number 2003-35205-12838).